Department of OBGYN

Kyle Orwig, Ph.D.

  • Professor, Department of Obstetrics, Gynecology & Reproductive Sciences

Education & Training

  • Senior Postdoctoral Fellow, University of Pennsylvania
  • Postdoctoral training, University of Kansas Medical Center
  • PhD, Biochemistry & Biophysics and Animal Sciences, Oregon State University

Representative Publications

Song HW, Bettegowda A, Lake BB, Zhao AH, Skarbrevik D, Babajanian E, Sukhwani M, Shum EY, Phan MH, Plank TD, Richardson ME, Ramaiah M, Sridhar V, de Rooij DG, Orwig KE, Zhang K, Wilkinson MF. The Homeobox Transcription Factor RHOX10 Drives Mouse Spermatogonial Stem Cell Establishment. 2016. Cell Reports. 1:149-64. [PUBMED]

Gassei K, Orwig KE.  Experimental methods to preserve male fertility and treat male factor infertility. 2016. Fertil Steril. 2:556-566. [PUBMED]

Gassei K, Valli H, Orwig KE. Whole-mount immunohistochemistry to study spermatogonial stem cells and spermatogenic lineage development in mice, monkeys, and humans. 2014. Methods Mol Biol. 1210:193-202. [PUBMED]

Dovey SL, Valli H, Hermann BP, Sukhwani M, Donohue J, Castro CA, Chu T, Sanfilippo JS, Orwig KE. 2013. Eliminating malignant contamination from therapeutic human spermatogonial stem cells. J Clin Invest 123(4):1833-43. [PUBMED]

Hermann BP, Sukhwani M, Winkler F, Pascarella JN, Peters KA, Sheng Y, Valli H, Rodriguez M, Ezzelarab M, Dargo G, Peterson K, Masterson K, Ramsey C, Ward T, Lienesch M, Volk A, Cooper DK, Thomson AW, Kiss JE, Penedo MC, Schatten GP, Mitalipov S, Orwig KE. 2012. Spermatogonial stem cell transplantation into rhesus testes regenerates spermatogenesis producing functional sperm. Cell Stem Cell 11(5):715-26. [PUBMED]


Clinical Interests/Research Interests

Research in the Orwig laboratory focuses on stem cells, germ lineage development, fertility and infertility.  Our progress investigating reproductive function in fertile individuals provides a basis for understanding the mechanisms of infertility caused by disease, medical treatments, genetic defects or aging.  Infertility impacts one in seven couples in the United States and can have a devastating impact on relationships and emotional well-being.  The Orwig lab is ideally located in Magee-Womens Research Institute and Magee-Womens Hospital of the University of Pittsburgh and is committed to translating lab bench discoveries to the clinic for diagnosis, prevention and treatment of infertility.

Orwig Laboratory Website:

Fertility Preservation Program: